RWJMS Flow Cytometry Core Facility
An important note to investigators: We request that users formally acknowledge the services of Flow Cytometry Core Facility in their publications, as per our policies. We suggest the following language: This research was supported by the Flow Cytometry Core Facility of Rutgers Robert Wood Johnson Medical School, a Shared Resource of The Rutgers Cancer Institute of New Jersey (P30CA072720) and NIH Shared Instrumentation Grant (1 S10 RR025468-01).
The Flow Cytometry Core Facility (FCCF) of Rutgers Robert Wood Johnson Medical School (RWJMS) is part of the Rutgers Cancer Institute of New Jersey Flow Cytometry/Cell Sorting Core Facility. We provide flow cytometry (a.k.a. "FACS"), cell sorting, and related services to investigators at Rutgers University, Princeton University, and other academic institutions, and to commercial users in the area.
The Core Facility provides instrumentation and expertise in flow cytometry and cell sorting. Located in the Rutgers Cancer Institute of NJ, we currently operate an FC-500 flow cytometer (2-lasers, 5-color detection) from Beckman-Coulter, and an ultra high-speed Influx cell sorter (4-lasers including UV, 12-colors) from BD Biosciences, with sterile capability and biohazard containment. We also are pleased to collaborate in experimental design, data analysis, and in the preparation of publications, grant applications, abstracts, and posters.
Samples are usually prepared by the investigator and brought to the Facility to be run with or without staff assistance. Flow cytometry applications include cell cycle analysis, apoptosis assays, proliferation assays, FRET, and determination of surface and intracellular markers. For sorting, cells from a wide variety of organisms (primary or cultured) can be sorted in bulk and by single cell deposition. A broad range of fluorescent markers and dyes can be utilized, including fluorescein (FITC), phycoerythrin (PE) and its tandems, Cy3, Cy5, Cy5.5, Cy7, APC and it's tandems, Alexa dyes, and Quantum dots; fluorescent proteins GFP, RFP, DsRed, and mCherry; propidium iodide, 7-AAD, and Hoechst stains. We currently accept samples only for research, but hope to add experimental clinical sorting.
The services provided by the FCCF are intended to help develop strategies to prevent, diagnose, and treat cancer and other genetic and infectious diseases, and to study the underlying biology of these conditions.
FACS: fluorescence activated cell sorting, FRET: fluorescence resonance energy transfer